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Leviticus 16

Lev. 16:31 KJV (With Strong’s)

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31
It shall be a sabbath
shabbath (Hebrew #7676)
intermission, i.e (specifically) the Sabbath
KJV usage: (+ every) sabbath.
Pronounce: shab-bawth'
Origin: intensive from 7673
q of rest
shabbathown (Hebrew #7677)
a sabbatism or special holiday
KJV usage: rest, sabbath.
Pronounce: shab-baw-thone'
Origin: from 7676
unto you, and ye shall afflict
`anah (Hebrew #6031)
to depress literally or figuratively, transitive or intransitive (in various applications, as follows)
KJV usage: abase self, afflict(-ion, self), answer (by mistake for 6030{/SI}}}6030{/SI}}/SI}}6030}6030{/SI}{/SI}), chasten self, deal hardly with, defile, exercise, force, gentleness, humble (self), hurt, ravish, sing (by mistake for 6030{/SI}}}6030{/SI}}/SI}}6030}6030{/SI}{/SI}), speak (by mistake for 6030{/SI}}}6030{/SI}}/SI}}6030}6030{/SI}{/SI}), submit self, weaken, X in any wise.
Pronounce: aw-naw'
Origin: a primitive root (possibly rather ident. with 6030{/SI}}}6030{/SI}}/SI}}6030}6030{/SI}{/SI} through the idea of looking down or browbeating)
your souls
nephesh (Hebrew #5315)
properly, a breathing creature, i.e. animal of (abstractly) vitality; used very widely in a literal, accommodated or figurative sense (bodily or mental)
KJV usage: any, appetite, beast, body, breath, creature, X dead(-ly), desire, X (dis-)contented, X fish, ghost, + greedy, he, heart(-y), (hath, X jeopardy of) life (X in jeopardy), lust, man, me, mind, mortally, one, own, person, pleasure, (her-, him-, my-, thy-)self, them (your)-selves, + slay, soul, + tablet, they, thing, (X she) will, X would have it.
Pronounce: neh'-fesh
Origin: from 5314
, by a statute
chuqqah (Hebrew #2708)
feminine of 2706, and meaning substantially the same
KJV usage: appointed, custom, manner, ordinance, site, statute.
Pronounce: khook-kaw'
for ever
`owlam (Hebrew #5769)
from 5956; properly, concealed, i.e. the vanishing point; generally, time out of mind (past or future), i.e. (practically) eternity; frequentatively, adverbial (especially with prepositional prefix) always
KJV usage: alway(-s), ancient (time), any more, continuance, eternal, (for, (n-))ever(-lasting, -more, of old), lasting, long (time), (of) old (time), perpetual, at any time, (beginning of the) world (+ without end). Compare 5331, 5703.
Pronounce: o-lawm'
Origin: or lolam {o-lawm'}
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J. N. Darby Translation

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31
A sabbath of rest shall it be unto you, and ye shall afflict your souls: it is an everlasting statute.