By nature we know separation from God!
In Christ we know separation to Him!
When Adam sinned in the Garden of Eden, the happy communion that he enjoyed with his God was immediately broken. Having received a conscience, realizing that he had sinned, he hid himself behind the trees of the garden in fear of the voice of God.
But for those of us who know Christ as Saviour, we have been brought into the closest and most blessed place of relationship possible. There must be practical separation from the world and from evil if we are going to enjoy that relationship. We are exhorted, “Be ye separate, saith the Lord” (2 Corinthians 6:17).